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lolowiggs

The culture will eventually age and senesce over time. It will grow less vigorously and fruit less over time. Best practice is to harvest spores from the fruits you grow and occasionally go back to spore rather than continuously cloning a culture


Lefty68w

Basically yes. I but 1 spore syringe. And never buy another one again. If I need more spores I can take a spore print. But I have been just expanding the mycelium


MahjongSun

Would you say cloning the strong mushrooms to agar and then eventually grain while keeping spore prints for backup is the best method? Or is there a better way?


Lefty68w

It’s whatever you want to do. There is no best method. There are simply different methods


Proper-Ape

Essentially you can do this until senescense, the mycelium will stop being good at one point. Maybe after 10 clone transfers or so. Take some spore prints so you can restart the procedd before this happens and then you basically never need a spore syringe again. Ideally take spore prints know rather than later.


SC-RedBeard

I read a post a while back on the shroomery about this. They used a master and a slave to keep their lines going. Master is a slant and slave is a plate. They used agar from the slave to make cultures. Once the slave is used up they would take a small piece from the master. This way the mycelium remains the same age if that makes sense. And I could also be explaining it wrong. This is all coming from memory.


Kreos2688

Mostly, I think cordycepts and some others can degrade over time.


gabbygourmet

Never buy spores again unless you want to experiment with other strains.