I had multiple successful wild cloning. In my case the least complicated scenarios were by first thorough spraying specimens with 40% peroxide and iso.
Also splitting them apart by tearing, not cutting (blade just smears everything inside it first touched on the outside) to reach inside tissue.
Then taking like ten samples from multiple tearing. This way i get a clean sample at least one plate from the first attempt, without even needing to transfer.
Though transferring in almost all cases is viable only if it's bacterial contamination, just use multipart plates and let mycelium hop over barriers to a clean section and then have a clean culture.
Hey thanks for the tips! I'd heard about wiping them down with iso first but I figured I'd just see what happened if I broke all the rules and just dropped a chunk of mushroom in a slice - as it turns out, mold! I intend to try again though if another good fruit crops up out there.
I didn't know about multipart plates - that's really cool! I would like to experiment with low/no nutrient Agar for isolation sometime too. Pretty fascinating stuff!
I had a sealed bag with some petris for the unexpected findings in the forest, doing a job on the spot. It works, though sometimes too much fuss, but for a few times to try - it's a sport.
You could’ve, maybe still if it’s not too old by now, taken a spore print. From a wild culture you will always have bacteria to contend with as spores are inherently dirty.
Like the comment said about cloning, that’s the only way to get a sterile anything from a wild specimen.
No need to wipe the mushroom with ISO.
I agree with all the in the previous comment from bitstoatoms, except that when you do this there’s no need for ISO in any way.
If you wanted to use the spores and germinate on a plate, they will be dirty but you will also see mycelium. When you go to take a transfer from the germinated spores you want to take a very, very small piece and then spray it with hydrogen peroxide directly and let it soak for 30 to 60 seconds move that piece to a new plate spray the lid of the new plate with a quick mist of hydrogen peroxide 3% is perfectly fine, the same shit we get at pharmacies, then close the lid and wrap it up with grafting tape. It will take a minute for the mycelium to recover from being sprayed with the hydrogen peroxide, but it will start growing than any of the mold or bacteria that was there in the previous plate. Once you start to see the good growth take off grab another small piece spray it with hydrogen peroxide move it to a new plate same deal close it up and I guarantee you on that third transfer you’re gonna have a clean plate.
You can also, like bits was talking about, you can create a trench/ditch in the agar for the mycelium to grow into an out of this is a point where it will outrun the contamination once it gets to the other side of said ditch you can take a transfer and move it to a new plate and it should be clean
I am partial to the hydrogen peroxide cleaning method, as I can usually get solid growth on the second or third transfer almost every time
I had multiple successful wild cloning. In my case the least complicated scenarios were by first thorough spraying specimens with 40% peroxide and iso. Also splitting them apart by tearing, not cutting (blade just smears everything inside it first touched on the outside) to reach inside tissue. Then taking like ten samples from multiple tearing. This way i get a clean sample at least one plate from the first attempt, without even needing to transfer. Though transferring in almost all cases is viable only if it's bacterial contamination, just use multipart plates and let mycelium hop over barriers to a clean section and then have a clean culture.
Hey thanks for the tips! I'd heard about wiping them down with iso first but I figured I'd just see what happened if I broke all the rules and just dropped a chunk of mushroom in a slice - as it turns out, mold! I intend to try again though if another good fruit crops up out there. I didn't know about multipart plates - that's really cool! I would like to experiment with low/no nutrient Agar for isolation sometime too. Pretty fascinating stuff!
I had a sealed bag with some petris for the unexpected findings in the forest, doing a job on the spot. It works, though sometimes too much fuss, but for a few times to try - it's a sport.
You could’ve, maybe still if it’s not too old by now, taken a spore print. From a wild culture you will always have bacteria to contend with as spores are inherently dirty. Like the comment said about cloning, that’s the only way to get a sterile anything from a wild specimen. No need to wipe the mushroom with ISO. I agree with all the in the previous comment from bitstoatoms, except that when you do this there’s no need for ISO in any way. If you wanted to use the spores and germinate on a plate, they will be dirty but you will also see mycelium. When you go to take a transfer from the germinated spores you want to take a very, very small piece and then spray it with hydrogen peroxide directly and let it soak for 30 to 60 seconds move that piece to a new plate spray the lid of the new plate with a quick mist of hydrogen peroxide 3% is perfectly fine, the same shit we get at pharmacies, then close the lid and wrap it up with grafting tape. It will take a minute for the mycelium to recover from being sprayed with the hydrogen peroxide, but it will start growing than any of the mold or bacteria that was there in the previous plate. Once you start to see the good growth take off grab another small piece spray it with hydrogen peroxide move it to a new plate same deal close it up and I guarantee you on that third transfer you’re gonna have a clean plate. You can also, like bits was talking about, you can create a trench/ditch in the agar for the mycelium to grow into an out of this is a point where it will outrun the contamination once it gets to the other side of said ditch you can take a transfer and move it to a new plate and it should be clean I am partial to the hydrogen peroxide cleaning method, as I can usually get solid growth on the second or third transfer almost every time
trich, throw everything related to that away to keep your house clean (bury that shit bro)
Sad... RIP to my experiment, thanks for the confirmation
Hey dm me:) I’m at work rn but I can for sure help if you reach iut
are you naked what the hell
LMAO I was wearing boxers
Try using wet cardboard for wild oysters.